Bacteriophage Based Assay to Detect Mycobacteria in the Sputum of Tuberculosis Patients
Early, efficient, and inexpensive methods for the detection of pulmonary tuberculosis (TB) are urgently needed for effective patient management as well as to interrupt transmission. The major factors influencing the clinical outcome and control of the transmission of TB from patients includes the low sensitivity of the conventional Ziehl-Neelsen method and the long time taken to obtain drug susceptibility data. One of the most promising approaches is the use of mycobacteriophage D29 to demonstrate the viable mycobacterium cells. The aim of this review was to document the role of mycobacteriophages, particularly mycobacteriophage D29 to detect M. tuberculosis in sputum samples from TB patients. The review was organized by collecting different published information and after core laboratory skills were acquired. Fast PlaqueTM is a rapid test which utilizes bacteriophage amplification technology for the detection of viable M. tuberculosis in clinical specimens. Taking BACTEC 460 TB culture as the gold standard, the sensitivity of Fast PlaqueTM test reported 87.5% that Ziehl-Neelsen microscopy can detect when the sample has > 104 bacilli/ml of sputum, but the Fast PlaqueTM test can detect 100-300 bacilli/ml of sputum. The diagnostic performance of the Fast PlaqueTM test is reported to be comparable with the GeneXepert test, especially on smear negative specimens (sensitivity of 65% versus 72.5%, respectively). Moreover, the cost is by far less than that of the other available tests, like culture or polymerase chain reaction (PCR). In conclusion, the Fast PlaqueTM test has a good potential for rapid diagnosis of M. tuberculosis. Thus it can be useful in the diagnosis of tuberculosis as an adjunct to sputum microscopy particularly in TB endemic and resource poor countries. In addition, Fast PlaqueTM-RIFTM offered a performance comparable to the gold standard proportion methods of rifampicin susceptibility testing.
Keywords
Authors
How to Cite
Download Citation
License
Copyright © 2014 The Author(s)
Similar Articles
- Getachew Ferede, Moges Tiruneh, Ebba Abate, Yitayih Wondimeneh, Belay Tessema, Uwe G Liebert, Distribution of Dengue Virus Serotype Based on Neutralization Assay in Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 13 No. 1 (2023)
- Mebrate Dufera, Hans C.Aass , Nega Berhe , Beyene Petros , Berhanu Erko, Cytokine Response in Mono and Malaria-S. mansoni Co-Infected Individuals in Fincha Sugar Estate, Western Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 9 No. 1 (2019)
- Debasu Damtie, Dilaferahu Abebe , Dawit Tatek , Baye Gelaw , A Three-Year Retrospective Study on The Prevalence of HBV, HCV, and HIV Among Blood Donors At The University Of Gondar Hospital Blood Bank, North West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 2 (2010)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Dessie Tegegne, Aschalew Gelaw, Demeke Endalamaw, Getachew Ferede, Baye Gelaw, The association between ABO blood groups and norovirus infections among patients suffering from diarrheal disease in Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 14 No. 1 (2024)
- Abebaw Bitew, Aschalew Gelaw, Yitayih Wondimeneh, Gizachew Yismaw, Ashenafi Alemu, Getachew esfaye, Mekonnen Teferi, Takele Abayneh, Molalegne Bitew, Markos Abebe, Biruk Yeshitela, Adane Mihret, Baye Gelaw, Evaluation of Crystal® VC Rapid Diagnostic Test kit to detect Vibrio cholerae from fecal samples in Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 15 No. 1 (2025)
- Baye Gelaw, Shimelis Assefa, Takele Teklu, Simon G/tsadik, Wubet Berhane, Meseret Alem, Yetayal Sheferaw, Audit on the Laboratory Diagnosis of Malaria Parasites at the University of Gondar Hospital Laboratory, Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 3 No. I (2010)
- Yeterefwork Tsige , Baye Gelaw, Marta Alemayhu, Anteneh Amsalu, The Prevalence Of Methicilin Resistant Staphylococcus Aureus And Associated Risk Factors Among Patients With Wound Infection At Dessie Referral Hospital, Northeast Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 8 No. 1 (2017)
- Baye Gelaw, Natalie J. Garton, Abraham Aseffa, Michael Richard , Molecular Characterization of Mycobacteria Causing Pulmonary Tuberculosis in Gondar, North-West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 6 No. 1 (2014)
- Baye Gelaw, The Role of Lipid Bodies in Mycobacterium Tuberculosis Infection , Ethiopian Journal of Health and Biomedical Sciences: Vol. 5 No. 1 (2012)
- Baye Gelaw, Monkeypox Virus Infection in Humans: Past and new potential threat at our doorstep , Ethiopian Journal of Health and Biomedical Sciences: Vol. 12 No. 2 (2022)