Comparison of Sysmex-Kx-21 with Manual Methods: Hemocytometery, Sahli-Hellige and Microhematocrite Methods in Quantitative Hematology Analysis
Background: The manual blood cell counting is currently replaced by automation hematology analyzers in many clinical laboratories. Sysmex is a discrete hematology analyzer designed for high-volume testing in clinical laboratories. Though automation is widely accepted and is on use in different health institutions in Ethiopia, it is not yet evaluated for precision and accuracy nor compared with manual methods. This study compared four manual hematology analysis methods with Sysmex kx-21.
Objective: To compare Sysmex-kx-21 hematology analyzer with manual hemocytometery (WBCs), Sahli-Hellige (hemoglobin concentration), microhematocrit centrifuge method (hematocrit or packed cell volume) and the differential white blood cell
(WBC) count methods.
Methods: The white blood (WBC) cell enumeration, differential WBC count, hemoglobin and hematocrit results of three laboratory technicians (two manually and one by using Sysmex- kx-21) was compared. Blood samples from 130 patients and 30 students were collected between May and June, 2008. Each sample was investigated manually and by the SYSMEX-KX-21 automation.
Data was registered and analyzed using Microsoft Excel 2003 and SPSS version 13 computer software programs.
Results: There was no significant difference in the total WBC count between the two manual readers and between the two manual readers and the automation. The results of the three readers strongly matched on the total WBC count and hemoglobin concentration. The result of the manual hematocrit readers was less correlated with the automation. The two manual readers were almost not correlated on the lymphocyte differential WBC count.
Conclusions: The overall correlation of the manual methods to the automation can be graded as good. Standardizing the automation, combined use of manual methods with automation at higher health institutions and the use of manual methods by the peripheral health unites are recommended.
Keywords
Authors
How to Cite
Download Citation
License
Copyright © 2020 The Author(s)
Similar Articles
- Bahiru Tenaw, Abebe Muche, Engidaw Fentahun , Abyot Endale , An Evaluation of Acute and Sub-Acute Toxicity of Methanolic Root Extract Of Myricasalicifolia A. Rich (Myricaceae) On the Histopathology of the Liver, the Kidney and Some Blood Parameters in Swiss Albino Mice , Ethiopian Journal of Health and Biomedical Sciences: Vol. 9 No. 1 (2019)
- Kassa Birhan, Yigzaw Kebede, Incidence of Tuberculosis and Associated Factors among Patients Attending the HIV Clinic at Felege-Hiwot Referral Hospital, Bahr Dar, Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 6 No. 1 (2014)
- Asressie M, Yigzaw K , Telake A, Survival and Predictors of the Survival of HIV-Infected Adult Patients Taking HAART at Hawassa University Referral Hospital , Ethiopian Journal of Health and Biomedical Sciences: Vol. 6 No. 1 (2014)
- Menberework Chanyalew, Abraham Aseffa, Morten Harboe, Joseph Olobo Okao, Asrat Hailu, Experimental Infections with Leishmania Aethiopica and Immune Response Analysis of Cercopithecus Aethiops , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 1 (2009)
- Debasu Damtie, Dilaferahu Abebe , Dawit Tatek , Baye Gelaw , A Three-Year Retrospective Study on The Prevalence of HBV, HCV, and HIV Among Blood Donors At The University Of Gondar Hospital Blood Bank, North West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 2 (2010)
- Sisay Getie, Abebe Genetu , Abebe A. Fola, Ligabaw Worku, Gebeyaw Getnet , Meseret Birhanie, Yitayih Wondimeneh, Getachew Ferede, Mengistu Endris, Agersew Alemu, Biniam Mathewos, Aberham Abere, Robert Burton, Dylan R Pillai, Preventive Measures and Diagnosis of Asymptomatic Malaria in Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 8 No. 1 (2017)
- Segen Tekle, Feleke Moges, Zemene Tigabu, Alem Getaneh, Bacterial Profile and Their Antibiotic Resistance Patterns from Blood Culture and Associated Risk Factors in Intensive Care Unit at the University Of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 10 No. 1 (2020)
- Baye Gelaw, Shimelis Assefa, Takele Teklu, Simon G/tsadik, Wubet Berhane, Meseret Alem, Yetayal Sheferaw, Audit on the Laboratory Diagnosis of Malaria Parasites at the University of Gondar Hospital Laboratory, Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 3 No. I (2010)
- Mequanint Bezie, Moges Tiruneh, Belay Anagaw, Mulat Dagnew, Bacterial Causes of Acute Febrile Illnesses and Their Antimicrobial Susceptibility Patterns in Adult Patients Attending Felege-Hiwot Referral Hospital, Northwest Ethiopia: A Cross Sectional Study , Ethiopian Journal of Health and Biomedical Sciences: Vol. 8 No. 1 (2017)
- Mebrate Dufera, Beyene Petros, Tekola Endeshaw, Hussien Mohammed, Afework Kassu, Opportunistic Intestinal Protozoan Parasites among HIV Positive Patients on Antiretroviral Therapy at Nekemte Hospital, West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 1 No. 1 (2008)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Debasu Damtie, Dilaferahu Abebe , Dawit Tatek , Baye Gelaw , A Three-Year Retrospective Study on The Prevalence of HBV, HCV, and HIV Among Blood Donors At The University Of Gondar Hospital Blood Bank, North West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 2 (2010)