Comparison of Sysmex-Kx-21 with Manual Methods: Hemocytometery, Sahli-Hellige and Microhematocrite Methods in Quantitative Hematology Analysis
Background: The manual blood cell counting is currently replaced by automation hematology analyzers in many clinical laboratories. Sysmex is a discrete hematology analyzer designed for high-volume testing in clinical laboratories. Though automation is widely accepted and is on use in different health institutions in Ethiopia, it is not yet evaluated for precision and accuracy nor compared with manual methods. This study compared four manual hematology analysis methods with Sysmex kx-21.
Objective: To compare Sysmex-kx-21 hematology analyzer with manual hemocytometery (WBCs), Sahli-Hellige (hemoglobin concentration), microhematocrit centrifuge method (hematocrit or packed cell volume) and the differential white blood cell
(WBC) count methods.
Methods: The white blood (WBC) cell enumeration, differential WBC count, hemoglobin and hematocrit results of three laboratory technicians (two manually and one by using Sysmex- kx-21) was compared. Blood samples from 130 patients and 30 students were collected between May and June, 2008. Each sample was investigated manually and by the SYSMEX-KX-21 automation.
Data was registered and analyzed using Microsoft Excel 2003 and SPSS version 13 computer software programs.
Results: There was no significant difference in the total WBC count between the two manual readers and between the two manual readers and the automation. The results of the three readers strongly matched on the total WBC count and hemoglobin concentration. The result of the manual hematocrit readers was less correlated with the automation. The two manual readers were almost not correlated on the lymphocyte differential WBC count.
Conclusions: The overall correlation of the manual methods to the automation can be graded as good. Standardizing the automation, combined use of manual methods with automation at higher health institutions and the use of manual methods by the peripheral health unites are recommended.
Keywords
Authors
How to Cite
Download Citation
License
Copyright © 2020 The Author(s)
Similar Articles
- Biniam Kefiyalew, Tezera Tadele, Zeleke Mekonnen, Binyam Tilahun, Bethelhem Anteneh, Alemayehu Teklu, Community engagement platforms for Compassionate Respectful and Caring health service: challenges and opportunities, in Sululta district, Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 12 No. 1 (2022)
- Dagnew Engdaw, Hameed Sulaiman, Seyoum Leta, Determining the Generation Rate and Composition of Solid Health Care Waste at Gondar University Hospital , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 1 (2009)
- Baye Gelaw, Natalie J. Garton, Abraham Aseffa, Michael Richard , Molecular Characterization of Mycobacteria Causing Pulmonary Tuberculosis in Gondar, North-West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 6 No. 1 (2014)
- Mulu Slute, Getu Debalkie, Kindie Fentahun, Sebsebie Tadesse, Kassahun Alemu, Perception of Climate Change and Associated Factors among Rural Dwellers of Gondar Zuria District, Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 9 No. 1 (2019)
- Abiyu Geta, Alemayehu Teklu, Kemal Jemal, Binyam Tilahun, Exploring barriers and enablers of compassionate and respectful care implementation in pre-service education in Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 12 No. 1 (2022)
- Aberham Abere, Abebe Genetu Bayih, Adane Derso, Dessie Abebaw, Ligabaw Worku, Mulugeta Aemero, Schistosoma mansoni in Lake Tana: A comprehensive assessment of parasite, snail vector, and associated factors at Gorgora, Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 13 No. 2 (2023)
- Adane Kebede, Endalkachew Dellie, Melak Jejaw, Misganaw Guadie Tiruneh, Getachew Teshale, Demiss Mulatu Gebru, Kaleb Assegid Demissie, Impact of Human Resources for Health flooding Strategy on Quality of ANC and Intrapartum care service in Ethiopia: A quasi-experimental pre-post study , Ethiopian Journal of Health and Biomedical Sciences: Vol. 15 No. 2 (2025)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Debasu Damtie, Dilaferahu Abebe , Dawit Tatek , Baye Gelaw , A Three-Year Retrospective Study on The Prevalence of HBV, HCV, and HIV Among Blood Donors At The University Of Gondar Hospital Blood Bank, North West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 2 (2010)