Comparison of Sysmex-Kx-21 with Manual Methods: Hemocytometery, Sahli-Hellige and Microhematocrite Methods in Quantitative Hematology Analysis
Background: The manual blood cell counting is currently replaced by automation hematology analyzers in many clinical laboratories. Sysmex is a discrete hematology analyzer designed for high-volume testing in clinical laboratories. Though automation is widely accepted and is on use in different health institutions in Ethiopia, it is not yet evaluated for precision and accuracy nor compared with manual methods. This study compared four manual hematology analysis methods with Sysmex kx-21.
Objective: To compare Sysmex-kx-21 hematology analyzer with manual hemocytometery (WBCs), Sahli-Hellige (hemoglobin concentration), microhematocrit centrifuge method (hematocrit or packed cell volume) and the differential white blood cell
(WBC) count methods.
Methods: The white blood (WBC) cell enumeration, differential WBC count, hemoglobin and hematocrit results of three laboratory technicians (two manually and one by using Sysmex- kx-21) was compared. Blood samples from 130 patients and 30 students were collected between May and June, 2008. Each sample was investigated manually and by the SYSMEX-KX-21 automation.
Data was registered and analyzed using Microsoft Excel 2003 and SPSS version 13 computer software programs.
Results: There was no significant difference in the total WBC count between the two manual readers and between the two manual readers and the automation. The results of the three readers strongly matched on the total WBC count and hemoglobin concentration. The result of the manual hematocrit readers was less correlated with the automation. The two manual readers were almost not correlated on the lymphocyte differential WBC count.
Conclusions: The overall correlation of the manual methods to the automation can be graded as good. Standardizing the automation, combined use of manual methods with automation at higher health institutions and the use of manual methods by the peripheral health unites are recommended.
Keywords
Authors
How to Cite
Download Citation
License
Copyright © 2020 The Author(s)
Similar Articles
- Baye Gelaw, Monkeypox Virus Infection in Humans: Past and new potential threat at our doorstep , Ethiopian Journal of Health and Biomedical Sciences: Vol. 12 No. 2 (2022)
- Aschalew Gelaw, Review on the epidemiology of astrovirus infections in East Africa and its public health significance , Ethiopian Journal of Health and Biomedical Sciences: Vol. 15 No. 1 (2025)
- Afework Kassu, Masayuki Fujino, Masako Nishizawa, Getahun Mengistu, Ermias Diro, Belete Ayele, Dereje Ketema, Feleke Moges, Andargachew Mulu, Gizachew Yismaw, Kahsay Huruy, Ebba Abate, Assefa Getachew, Moges Tiruneh, Yared Wondmikun, Wataru Sugiura, Fusao Ota, Levels of serum HIV-1 RNA viral load in tuberculosis patients with or without intestinal parasites during treatment of tuberculosis in Gondar, Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 1 No. 1 (2008)
- Getu Degu Alene, Harmful Traditional Health Practices: A Cross – Sectional Survey among Under-Five Children in Dembia District, North-West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 2 (2010)
- Jemal Ali, Huda Mohammed, Adugna Abera, Yohannes Mengistu, Beverley Singh, Adrian Puren, Dawit Wolday, Comparison of the Bed-Igg Captured EIA HIV-1 Incidence Assay with the Who-Criteria for Identifying Recently HIV Infected Individuals for Enrollment in the HIVDR-TS , Ethiopian Journal of Health and Biomedical Sciences: Vol. 5 No. 1 (2012)
- Mihretu Jegnie Jegnie, Yared Asmare, Abebe Muche, Anthropometric Measurements of Jugular Foramen in Ethiopian Dried Adult Skulls , Ethiopian Journal of Health and Biomedical Sciences: Vol. 10 No. 1 (2020)
- Moges Tadesse, Takele Tadesse, Hardeep Rai Sharma, Mengsha Admassu, Prevalence of Needle-Stick Injuries and Associated Factors among Health Care Workers at Hawassa Town, South Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 2 (2010)
- Berihun Megabiaw , Belaynew Wasie, Nikki L. Rogers, Malnutrition among HIV-Positive Children at Two Referral Hospitals in Northwest Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 5 No. 1 (2012)
- Amare Minyihun, Masrie Genet, Binyam Tilahun, How to strengthen the Monitoring and Evaluation System to improve Compassionate, Respectful, and Caring services in Ethiopia: An implementation study , Ethiopian Journal of Health and Biomedical Sciences: Vol. 12 No. 1 (2022)
- Melsew Yihunie Belay, Getahun Mengistu, Adugnaw Zeleke Alem, Tilahun Yemanu Birhan, Time to Recovery from Undernutrition and Its Predictors among Patients with Multidrug-Resistant Tuberculosis in Addis Ababa, Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 13 No. 2 (2023)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Debasu Damtie, Dilaferahu Abebe , Dawit Tatek , Baye Gelaw , A Three-Year Retrospective Study on The Prevalence of HBV, HCV, and HIV Among Blood Donors At The University Of Gondar Hospital Blood Bank, North West Ethiopia , Ethiopian Journal of Health and Biomedical Sciences: Vol. 2 No. 2 (2010)