Audit on the Laboratory Diagnosis of Malaria Parasites at the University of Gondar Hospital Laboratory, Northwest Ethiopia

Baye Gelaw , Shimelis Assefa , Takele Teklu , Simon G/tsadik , Wubet Berhane , Meseret Alem , Yetayal Sheferaw
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DOI: https://doi.org/10.20372/ejhbs.v3iI.172
Submitted: 2021-04-26 00:00:00
Published: 2021-06-17

Background: Malaria remains a heavy burden on tropical communities. In Ethiopia 65% of the population is living in 75% of the country at risk of malaria. Following correct laboratory procedures and identifying malaria parasites are of paramount importance in the laboratory diagnosis of malaria parasites. The aim of this audit was to assess the quality assurance system of malaria diagnosis in the University of Gondar (UOG) hospital against the established standards for the audit.
Methods: The clinical audit was conducted using a structured questionnaire, direct observation on laboratory activities, and re-examining the diagnosed slides confidentially. Standards were set for blood collection, the staining of blood films, the quality of the blood films, and reporting systems.
Results: Of the 214 blood samples examined for malaria parasites, 66 were true positives, 0 false positive, 7 false negatives and 141 true negative. Moreover, the wiping of the first drop of blood during capillary puncture, failure to filter Wright's staining solution on daily bases, and failure in the use of high- power objective during microscopy were among the highly violated standards with few areas of good practices.
Conclusion: In this audit, the false negative rate of malaria parasite microscopic examination in the UOG Hospital Laboratory which was 5% was significant. Replacing Wright's staining solution by Giemsa’s stain, preparing SOPs, and establishing internal quality control programs are recommended.

Keywords

Audit standard malaria blood film microscopy

Authors

Baye Gelaw

Department of Laboratory Technology, College of Medicine and Health Sciences, University of Gondar, P.O.Box 196

Shimelis Assefa

Department of Laboratory Technology, College of Medicine and Health Sciences, University of Gondar, P.O.Box 196

Takele Teklu

Department of Laboratory Technology, College of Medicine and Health Sciences, University of Gondar, P.O.Box 196

Simon G/tsadik

Department of Laboratory Technology, College of Medicine and Health Sciences, University of Gondar, P.O.Box 196

Wubet Berhane

Department of Laboratory Technology, College of Medicine and Health Sciences, University of Gondar, P.O.Box 196

Meseret Alem

Department of Laboratory Technology, College of Medicine and Health Sciences, University of Gondar, P.O.Box 196

Yetayal Sheferaw

Department of Laboratory Technology, College of Medicine and Health Sciences, University of Gondar, P.O.Box 196

How to Cite

Baye Gelaw, Shimelis Assefa, Takele Teklu, Simon G/tsadik, Wubet Berhane, Meseret Alem, Yetayal Sheferaw (2021-06-17). Audit on the Laboratory Diagnosis of Malaria Parasites at the University of Gondar Hospital Laboratory, Northwest Ethiopia. Ethiopian Journal of Health and Biomedical Sciences, Vol. 3 No. I (2010), 53-58. https://doi.org/10.20372/ejhbs.v3iI.172

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Copyright © 2021 The Author(s)

Copyright & Licensing © Year The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0) , which permits unrestricted use; sharing (copying and redistributing the material in any medium or format); and adaptation (remixing, transforming, and building upon the material for any purpose), provided the original author(s) and the source are properly cited/credited and a link to the license is provided. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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